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caspase 12 inhibition  (R&D Systems)


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    R&D Systems caspase 12 inhibition
    Caspase 12 Inhibition, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+atad+fmk/Caspase-12+Inhibitor+Z-ATAD-FMK/pm39437937-42-1-13
    Average 92 stars, based on 13 article reviews
    caspase 12 inhibition - by Bioz Stars, 2026-11
    92/100 stars

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    other:

    Article Title: Cancer markers and methods of use thereof
    Article Snippet: Membrane was blocked with 5% nonfat milk-supplied TBST for 30 min before probing with the anti-caspase-3 antibody that recognizes either procaspase-3 or cleaved/active form of caspase-3 (1:1,000 dilution; Abcam), followed by incubation with HRP-conjugated anti-rabbit antibody (1:5,000 dilution; Jackson ImmunoResearch) for 90 min.

    shRNA:

    Article Title: Stage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells
    Article Snippet: .. Cells were treated with or without β3GalT5 shRNA lentivirus (multiplicity of infection: 5), Z-DEVD-FMK (caspase-3 inhibitor) (50 μM and 100 μM; R&D Systems), Z-IETD-FMK (caspase-8 inhibitor), Z-LEHD-FMK (caspase-9 inhibitor), or Z-ATAD-FMK (caspase-12 inhibitor) (100 μM; R&D Systems) at 10 5 cells per mL as described. .. Three days later, cells were washed with PBS and incubated with allophycocyanin (APC)-conjugated annexin V (1:40 dilution; BD Biosciences) in binding buffer (0.01 M Hepes, 0.14 M NaCl, 2.5 mM CaCl 2 ) for 15 min on ice and then subjected to flow cytometric analysis.

    Infection:

    Article Title: Stage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells
    Article Snippet: .. Cells were treated with or without β3GalT5 shRNA lentivirus (multiplicity of infection: 5), Z-DEVD-FMK (caspase-3 inhibitor) (50 μM and 100 μM; R&D Systems), Z-IETD-FMK (caspase-8 inhibitor), Z-LEHD-FMK (caspase-9 inhibitor), or Z-ATAD-FMK (caspase-12 inhibitor) (100 μM; R&D Systems) at 10 5 cells per mL as described. .. Three days later, cells were washed with PBS and incubated with allophycocyanin (APC)-conjugated annexin V (1:40 dilution; BD Biosciences) in binding buffer (0.01 M Hepes, 0.14 M NaCl, 2.5 mM CaCl 2 ) for 15 min on ice and then subjected to flow cytometric analysis.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Soluble Fibrinogen Triggers Non-cell Autonomous ER Stress-Mediated Microglial-Induced Neurotoxicity
    Article Snippet: .. TGF-β and IL-6 ELISA kits, z-VAD-FMK and z-ATAD-FMK were from R&D Systems (Abingdon, United Kingdom). .. Caspase 12 (FITC-ATAD-FMK) activity kits were from Promokine (Heidelberg, Germany) and caspase 3/7 (FAM-DEVD-FMK) activity kits were from Millipore (Watford, United Kingdom).



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    The activity of calpain, <t>caspase-12,</t> caspase-9, and caspase-3 is increased on 8 day of NGF induction in pEGFP-Peripherin cells compared to PC cells . (A) Western blot analysis showing increased activation of caspase-12 (42 kD), caspase-9 (40 kD and 38 kD), and caspase-3 (19 kD and 17 kD) on later stage of NGF induction in pEGFP-Peripherin cells compared to PC cells. (B-E) Density of the bands for the calpain BDP (B), active caspase-12 (C), active caspase-9 (D), or active caspase-3 (E) relative to the β-actin band. The values are presented as the mean ± SEM for three experiments in each group. *p < 0.05, **p < 0.01 vs. control PC12 cells.
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    The activity of calpain, <t>caspase-12,</t> caspase-9, and caspase-3 is increased on 8 day of NGF induction in pEGFP-Peripherin cells compared to PC cells . (A) Western blot analysis showing increased activation of caspase-12 (42 kD), caspase-9 (40 kD and 38 kD), and caspase-3 (19 kD and 17 kD) on later stage of NGF induction in pEGFP-Peripherin cells compared to PC cells. (B-E) Density of the bands for the calpain BDP (B), active caspase-12 (C), active caspase-9 (D), or active caspase-3 (E) relative to the β-actin band. The values are presented as the mean ± SEM for three experiments in each group. *p < 0.05, **p < 0.01 vs. control PC12 cells.
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    The activity of calpain, caspase-12, caspase-9, and caspase-3 is increased on 8 day of NGF induction in pEGFP-Peripherin cells compared to PC cells . (A) Western blot analysis showing increased activation of caspase-12 (42 kD), caspase-9 (40 kD and 38 kD), and caspase-3 (19 kD and 17 kD) on later stage of NGF induction in pEGFP-Peripherin cells compared to PC cells. (B-E) Density of the bands for the calpain BDP (B), active caspase-12 (C), active caspase-9 (D), or active caspase-3 (E) relative to the β-actin band. The values are presented as the mean ± SEM for three experiments in each group. *p < 0.05, **p < 0.01 vs. control PC12 cells.

    Journal: Journal of Biomedical Science

    Article Title: A neuronal death model: overexpression of neuronal intermediate filament protein peripherin in PC12 cells

    doi: 10.1186/1423-0127-19-8

    Figure Lengend Snippet: The activity of calpain, caspase-12, caspase-9, and caspase-3 is increased on 8 day of NGF induction in pEGFP-Peripherin cells compared to PC cells . (A) Western blot analysis showing increased activation of caspase-12 (42 kD), caspase-9 (40 kD and 38 kD), and caspase-3 (19 kD and 17 kD) on later stage of NGF induction in pEGFP-Peripherin cells compared to PC cells. (B-E) Density of the bands for the calpain BDP (B), active caspase-12 (C), active caspase-9 (D), or active caspase-3 (E) relative to the β-actin band. The values are presented as the mean ± SEM for three experiments in each group. *p < 0.05, **p < 0.01 vs. control PC12 cells.

    Article Snippet: When used, the selective calpain inhibitor calpeptin (20 μM) (Calbiochem, La Jolla, CA), the caspase-9 inhibitor Ac-LEHD-CMK (20 μM) (Calbiochem), the caspase-12 inhibitor Z-ATAD-FMK (2 μM) (MBL, Nagoya, Japan) was added to the medium on day 6, with 1% DMSO being used as the vehicle control.

    Techniques: Activity Assay, Western Blot, Activation Assay

    Effects of calpain, caspase-9, and caspase-12 inhibitors on the survival of pEGFP-Peripherin cells . pEGFP-Peripherin cells were treated for 48 h with 20 μM calpeptin (calpain inhibitor), 20 μM Ac-LEHD-CMK (caspase-9 inhibitor), or 2 μM Z-ATAD-FMK (caspase-12 inhibitor) starting on day 6 of NGF induction. (A) Proteins were analyzed by Western blotting. Caspase-3 activity was detected by levels of the 120 kD caspase-3 breakdown product of α-fodrin (caspase-3 BDP). UT = untreated group. Equal loading of protein was confirmed using β-actin. (B) Cell viability was evaluated using the XTT assay. The data are presented as the mean ± SEM for eight experiments in each group. ** p < 0.01 versus untreated group. (C) The mitochondria membrane potential was evaluated by the ability of the cells to take up the fluorescent dye TMRE. Data are presented as the mean ± SEM for twelve experiments in each group. * p < 0.05 versus the untreated group.

    Journal: Journal of Biomedical Science

    Article Title: A neuronal death model: overexpression of neuronal intermediate filament protein peripherin in PC12 cells

    doi: 10.1186/1423-0127-19-8

    Figure Lengend Snippet: Effects of calpain, caspase-9, and caspase-12 inhibitors on the survival of pEGFP-Peripherin cells . pEGFP-Peripherin cells were treated for 48 h with 20 μM calpeptin (calpain inhibitor), 20 μM Ac-LEHD-CMK (caspase-9 inhibitor), or 2 μM Z-ATAD-FMK (caspase-12 inhibitor) starting on day 6 of NGF induction. (A) Proteins were analyzed by Western blotting. Caspase-3 activity was detected by levels of the 120 kD caspase-3 breakdown product of α-fodrin (caspase-3 BDP). UT = untreated group. Equal loading of protein was confirmed using β-actin. (B) Cell viability was evaluated using the XTT assay. The data are presented as the mean ± SEM for eight experiments in each group. ** p < 0.01 versus untreated group. (C) The mitochondria membrane potential was evaluated by the ability of the cells to take up the fluorescent dye TMRE. Data are presented as the mean ± SEM for twelve experiments in each group. * p < 0.05 versus the untreated group.

    Article Snippet: When used, the selective calpain inhibitor calpeptin (20 μM) (Calbiochem, La Jolla, CA), the caspase-9 inhibitor Ac-LEHD-CMK (20 μM) (Calbiochem), the caspase-12 inhibitor Z-ATAD-FMK (2 μM) (MBL, Nagoya, Japan) was added to the medium on day 6, with 1% DMSO being used as the vehicle control.

    Techniques: Western Blot, Activity Assay, XTT Assay